Where
Building 60, Room 162
National Institutes of Health
9000 Rockville Pike Bethesda, MD 20892
Driving Directions
BioTech 54: Making Cardiomyocytes from iPSCs
August 22 - 26, 2016
FAES Biotechnology Training Courses at the
National Institutes of Health
Registration occurs on a first-come, first-served basis. The deadline for registration is one week before the first day of the course. If you are unable to register before the deadline, please email: training@faes.org or call 301-496-7977 for space availability.
NIH employees paying with the signed vendor copy of the SF-182 form, please email: registration@faes.org
Often late stage clinical trials are terminated due to cardiotoxicity. There is
great need to develop proper screens that are predictive of human clinical
response to medications. This course will cover numerous applications using
cardiomyocytes. The lectures will cover cardiac development and cardiac diseases which provides the necessary background for this course in appreciating how stem cells can be differentiated from iPSCs and be used to develop disease in a dish models as well as screens to monitor specific cardiac phenotypes such as arrhythmia and cardiac toxicity. Lectures will also cover the methodology to drive differentiation of iPSCs toward cardiac lineages and the development of cardiac reporter lines that will be useful for screening applications.
The laboratory exercises will include basic handling of cardiomyocytes
and then delve into discovery techniques that focus on disease modeling and
phenotypic screening for small molecule therapeutics. Lab exercises will
conclude with providing exposure to transfection techniques as well as assays for proarrhythmia and toxicity.
Lecture Topics
Cardiac Development and Disease; iPS and ESC: Methodology for Differentiation of Cardiomyoctyes from ESC and iPSC; iPS and ESC: Overview on Benefits and Limitations of iPSC/ESC-derived Cardiomyocytes; Characterisation and Validation of hESC Derived Cardiomyocytes; High Content Screening for Cardiotoxicity of Anti-Cancer Drugs in hESC Derived Cardiomyocytes; Bioenergetic Modulation of Kinase Inhibitor Cardiotoxicity in hESC Derived Cardiomyocytes; Cardiac angiogenesis and vascular biology; Cardiac Physiology; Cardiac Electrophysiology and Functional Assays; Functional Improvement Following Cell Therapy for Ischemic Myocardium.
Laboratory Topics
Cardiac differentiation from iPSCs ; Basic Handling: Thawing and Plating iPSC-derived Cardiomyoctyes; Discovery Techniques; MEA Demonstration; xCelligance Demonstration; PART I (Transfecting iCell Cardiomyoctyes with one or a Combination of the Following: (a) Fluorescent Protein Marker and Subsequent Analysis via Flow for Transfection Efficiency, Luciferase Reporter, Most Likely CRE-Luciferase and Then Induction and Read-out of the System, (c) siRNA to Knock Down Housekeeping Gene and then Quantify Via Flow or HCl or Knock Down Ion Channels and Provide a Functional Readout); Compound dose response curves; Transfection and reporter assays; Organelle Toxicity; Discovery Techniques PART II: Disease Modeling and Phenotypic Screening for Small Molecule Therapeutics (Hypertrophy With Protein Based HCl Readout and a Phenotypic Screen with 6 Compounds to Look for Amelioration of the Pathology); Reporter Assays; GE Cytell Demonstration: Imaging (GE Lab); Organelle Toxicity; Field Trip to NCATS, Lecture on High Content Screening Methods.
General Training Rate
$1,795.00
Discounted Training Rates
$695.00 - NIH Trainees (fellows, postdocs, postbacs - NIH ONLY)
$1,295.00 - NIH Community
$1,595.00 - Academia, Government, Military
Comprehensive Binder
Contains all notes, protocols, and reference material needed. No additional books are required.
Credit
Although no grades are given for BioTech Courses, each participant will receive Continuing Education Units (CEUs) based on the number of contact hours. Upon completion of this 5-day program, each participant will receive 3.5 CEUs. Upon completion of each workshop, a certificate is issued.
Refund Policy
100% Refund or 100% Full Workshop Credit Available
Written Notification: 14 or more days prior to start of program.
50% Refund or 100% Full Workshop Credit Available Written Notification: 4 to 13 days prior to start of program.
0% Refund, 100% Full Workshop Credit Available
3 days or less prior to start of program.
Notification
- All cancellations must be received in writing via email to Ms. Carline Coote at ccoote@mail.nih.gov
- Cancellations received after hours are time marked for the following day.
- In regards to a full or partial refund or a full workshop credit, you must indicate which option you are selecting at the time of your written cancellation.
- All refund payments will be processed by the start of the initial workshop.
Suggested Accommodations
The Double Tree Hotel & Executive Center