FAES@NIH
Where
National Institutes of Health
Building 60, Room #162 9000 Rockville Pike Bethesda, MD 20892
Contact
Registration Information
Conference and Training Team
Telephone: 301-496-7977
Email: registration@faes.org
BioTech 23: Flow Cytometry : Principles
and Methods
May 3-6, 2016
Biotechnology Training Courses at the National Institutes of Health (NIH)
If you are an NIH employee paying with the signed vendor copy of the SF-182 form, please email: registration@faes.org
This four day lecture/laboratory workshop will cover various applications of
flow cytometry in basic research. Selected lecture topics will provide a broad
background about the functions of a flow cytometer; choice of fluorochromes;
data analysis and presentation; technical protocols for flow cytometric
procedures and troubleshooting during data acquisition and analysis.
Lecture and Laboratory Topics
Basic Setup of Flow Cytometers; Principle of Cell Sorting; Parameters for Sorting; Alternative Separation Techniques (Including Magnetic Bead Separation MACS); Choice of Fluorochromes Depending on the type of Instrument and Application; Parameters of Analysis, Data Analysis and Interpretation; In-depth Discussion of Examples of Various Flow Cytometric Assays (e.g. Cell Cycle Analysis, Cell Activation, Apoptosis, Immunology, Hematology, Biochemistry with Methodical Protocols); Multi-color Analysis (With Special Emphasis on How to Combine Flurochromes and Fluorescence Compensation); Choosing the Right Controls for Data Analysis (Isotype Controls; FMO controls; Blocking with Antibodies); Hands-on Laboratory with 4- and 8-color staining (BD Accuri and MACSQuant Analyzer); Data Workshop; Hands-on Data Analysis with samples From Cell Cycle Analysis ; Apoptosis; Multi-Color Surface Staining; Proliferation using Bio-Tracers; Software training: Introduction to FlowJo Cytometry Analysis Software.
General Training Rate
$1,595.00
Discounted Training Rates $495.00 - NIH Trainees (fellows, postdocs, postbacs - NIH ONLY) $1,095.00 - NIH Community $1,295.00 - Academia, Government, Military
Comprehensive Binder
Contains all notes, protocols, and reference material needed. No additional books are required.
Credit Although no grades are given for courses, each participant will receive Continuing Education Units (CEUs) based on the number of contact hours. Upon completion of the course each participant will receive a certificate, showing completion of the workshop and 2.8 CEUs.
Refund Policy 100% tuition refund or 100% full workshop credit is available for registrations cancelled 14 or more days prior to the start of the workshop.
50% tuition refund or 100% full workshop credit is available for registrations cancelled between 4 to 13 days prior to the start of the workshop.
0% refund, 100% full workshop credit is available for registrations cancelled 3 days or less prior to the start of the workshop.
Notification
- All cancellations must be received in writing via email to Ms. Carline Coote at ccoote@mail.nih.gov.
- Cancellations received after hours are time marked for the following day.
- In regards to a full or partial refund or a full workshop credit, you must indicate which option you are selecting at the time of your written cancellation.
- All refund payments will be processed by the start of the initial workshop.
Suggested Accommodations
The Double Tree Hotel & Executive Center