Biotechnology Training Courses at the National Institutes of Health (NIH)  

Global transcription patterns as well as nucleotide polymorphisms can now be readily evaluated using DNA microarrays. This functional genomics technology offers a powerful, non biased approach to the analysis of molecular mechanisms associated with any type of physiological change in a cell. Moreover, DNA microarrays have become a prominent technological tool for genotype characterization. This lecture and laboratory course will show how DNA microarrays can be used for different applications in the biomedical sciences and will introduce hands-on training to a complex yet exciting technology.

Lecture Topics 
Overview of DNA microarrays; Transcriptional analysis from single genes to whole genomes; Types and applications of microarrays; Use of microarrays for genotyping; comparative genome hybridization providing a molecular karyotype of the genome; Fabrication of DNA microarrays; microarray data analysis; Concepts of microarray bioinformatics; Dove-tailing expression data to genome sequences and other types of functional genomics; genotyping and resequencing using genechip technology; high throughput profiling platforms: development of tools for diagnosis and management of disease; introduction of new technologies – microfluidics-generated microarrays with in situ synthesized probes, Illumina bead technology.

Laboratory Topics
Isolation of total RNA; purification and quantitation of mRNA; fluorescent labeling of cDNA; Hybridization of cDNA to DNA arrays; Scanning and data collection; Analysis and display of data using clustering programs; Validation of microarray expression analysis by real-time and conventional RT-PCR.

Registration Fees
$595.00 - NIH Students (fellows, post docs, post bacs)
$1,195.00 - NIH Employees/Government Employees/Academia  
$1,795.00 - All Others

Related BioTech Course
BioTech 34: Advance Microarray Analysis and Pathway Integration

Comprehensive Binder Included 
Contains all notes, protocols, and reference material needed. No additional books are required. 

Academic Credit
Although no grades are given for BioTech Courses, each participant will receive Continuing Education Units (CEUs) based on the number of contact hours. Upon completion of this 5-day program, each participant will receive 3.5 CEUs. Upon completion of each workshop, a certificate is issued.

Refund Policy
1.  100% tuition refund is available for registrations cancelled 14 or more days prior to the start of the workshop. 
2.  50% tuition refund is available for registrations cancelled between 4 to 13 days prior to the start of the workshop. Instead of a partial refund, you may elect to take a full workshop credit, which can be applied to another BioTech program of similar length. 
3.  No refund is available for registrations cancelled 3 days or less prior to the start of the workshop.  A full workshop credit will be available to you, which can be applied to another BioTech program of similar length.
All cancellations must be received in writing via fax to the FAES office, Attn. Ms. Carline Coote; BioTech Registrar 301-402-0174 during normal business hours (8:30 – 4:30 EST).  Those received after hours are time marked for the following day.  In the case of situation numbers 2 or 3, you must indicate whether you are opting for the full credit alternative at the time of written cancellation. In both cases, all payments will be processed by the start of the initial workshop

Accommodation