FAES@NIH
Where
Bldg. 60, Room 162, NIH, Bethesda, MD10 Center Drive
Room 1N241
Bethesda, MD 20892
Driving Directions
Contact
Workshop Information
Conference and Training Team
Telephone: 301-496-7977
Email: registration@faes.org
Biotechnology Training Courses at the National Institutes of Health (NIH)
Laser Microdissection systems allow for the procurement of specific populations of cells from tissue and cytology and live cell culture samples containing heterogeneous populations of cells. The specificity of analyses is therefore much more representative of the disease process being studied. This approach to microdissection ensures that biological molecules, such as RNA and DNA and proteins, remain undamaged during the microdissection process. Downstream molecular analysis of these molecules produces accurate and assured results that have led to over 2,000 peer-reviewed publications by independent researchers.
In this five-day training program, participants learn to prepare tissue specimens for microdissection, then select and acquire homogenous cell populations using the mmi-CellCut, Leica LMD, Arcturus XT, and PALM microdissection systems. Instruction emphasizes operation of these LM systems, appropriate tissue handling and sample preparation for subsequent DNA, RNA or protein analysis, and methods for proper molecular extraction. Lecture and detailed instructions to prepare samples for several downstream molecular analyses are presented.
Lecture and Laboratory Topics
Overview of Laser Microdissection Technology (History, Theory, Applications); Tissue Sample and Slide preparation; Project set up and QC of microdissected samples, genomic analysis, mRNA analysis including quantitative RT-PCR gene expression analysis and microarrays from microdissected tissue samples, microRNA analysis from microdissected samples, and proteomic analysis from frozen and formalin-fixed, paraffin-embedded microdissected samples using Mass Spec. Special microdissection techniques such as immuno-guided LM and live cells dissections will be also covered. The lectures also include examples of actual scientific and clinical applications of LM-based studies. The hands on sessions include tissue slide preparation and histology review for LM, and practice on Arcturus XT, PALM, Leica and mmi-CellCut platforms.
Training Rate*
$1,795.00
*Multiple Registration Discount Available
Discounted Training Rates
$595.00 - NIH Trainees (fellows, postdocs, postbacs - NIH ONLY)
$1,295.00 - NIH Community
$1,595.00 - Academia, Government, Military
Comprehensive Binder
Contains all notes, protocols, and reference material needed. No additional books are required.
Credit
Although no grades are given for BioTech Courses, each participant will receive Continuing Education Units (CEUs) based on the number of contact hours. Upon completion of this 5-day program, each participant will receive 3.5 CEUs. Upon completion of each workshop, a certificate is issued.
Refund Policy
1. 100% tuition refund is available for registrations cancelled 14 or more days prior to the start of the workshop.
2. 50% tuition refund is available for registrations cancelled between 4 to 13 days prior to the start of the workshop. Instead of a partial refund, you may elect to take a full workshop credit, which can be applied to another BioTech program of similar length.
3. No refund is available for registrations cancelled 3 days or less prior to the start of the workshop. A full workshop credit will be available to you, which can be applied to another BioTech program of similar length.
All cancellations must be received in writing via fax to the FAES office, Attn. Ms. Carline Coote; BioTech Registrar 301-480-2003 during normal business hours (8:30 – 4:30 EST). Those received after hours are time marked for the following day. In the case of situation numbers 2 or 3, you must indicate whether you are opting for the full credit alternative at the time of written cancellation. In both cases, all payments will be processed by the start of the initial workshop.
Suggested Accommodations
The Double Tree Hotel & Executive Center