Biophysical Methods for Protein Interactions  

This workshop will review strategies for hydrodynamic and thermodynamic analysis of sedimentation equilibrium and sedimentation velocity analytical ultracentrifugation (AUC), dynamic light scattering (DLS), isothermal titration calorimetry (ITC), and fluorescence spectroscopy for the characterization of biological macromolecules in solution.  The workshop will start on Monday morning and end on Friday at noon. On the first day, we will provide an introduction into the experimental aspects of AUC, DLS and ITC. This part will take place in the laboratory. We will review the basic operation of the instruments, choice of buffers, and detection systems, and experimental design dependent on the system under study. For AUC, assembling the AUC cells and operating the XLA/I will be discussed in detail. The following days will cover many advanced topics of theory, data analysis and experimental setup. These lectures are accompanied by practical computer exercises with the software. The workshop is aimed at researchers with at least limited experience in AUC, DLS and/or ITC. An integral part of the workshop is the presentation by the participants of their systems of interest and informal discussion of data.

Target Audience: 

The workshop is aimed at researchers with at least limited experience in AUC, DLS, ITC, and/or fluorescence techniques for characterizing biomacromolecules.

Lecturers: 

Andrea Balbo - Bioengineering and Physical Science Shared Resource, NIBIB;

Rodolfo Ghirlando, Ph.D. - Laboratory of Molecular Biology, NIDDK;                  

Grzegorz Piszczek, Ph.D.  - Biophysics Core, NHLBI,

Peter Schuck, Ph.D.  - Dynamics of Macromolecular Assembly, LCIMB, NIBIB;

Huaying Zhao, Ph.D. - Dynamics of Macromolecular Assembly Section, LCIMB, NIBIB

Registration Fees:

$1295.00 - Industry Participants (Pharma, analytical and commerical affiliate employees)

$895.00 - Academic Participants (P.i's, post docs, professors, staff scientists,etc)

$495.00 - Active Student Participants (Phd students, post-bacIRTAs pending grad or med   school)  Documentation is Required.

Suggested Accommodations:

American Inn of Bethesda

A discounted rate of $119.00 per night (single or double) has been arranged for attendees at the American Inn of Bethesda.  You may reserve your room online at https://res.windsurfercrs.com/bbe/page2.aspx?pcode=52340&propertyid=9666&nights=1&checkin=12/08/2014&nights=5&group=AUC 

 or call the hotel directly at 301-656-9300 and ask for the AUC Group Rate. Complimentary  services includes:  free hot continental breakfast, wireless printing throughout hotel, and use of 24 hour business center.

The Double Tree Hotel & Executive Center  

Bethesda Hyatt Hotel 

Bethesda Court Hotel

Bethesda Marriott Hotel 

Cancellations and Refund
Refunds will be issued for all cancellations, received in writing, on or before December 1, 2014.    Please contact the training office at (301) 594-8985 or via email, training@faes.org, if you wish to cancel your registration.  No refunds will be issued after December 3, 2014.  

Image courtesy of the National Institute of Health/Department of Health and Human Services. Use of image does not imply endorsement of any activity or product by the National Institutes of Health/Department of Health and Human Services.