Complimentary Bioprocess Workshop

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Eppendorf invites you to be our guest at a complimentary bioprocess workshop where attendees will benefit as we present and discuss information on fermentation process scale-up and cell culture scale-up using stirred-tank single-use bioreactors.  Our integrated portfolio of bioprocess systems, software and consumables will be on hand for demonstration including the DASbox® Mini Bioreactor System, the BioFlo® 120 and BioFlo® 320 Controllers, as well as our full line of Single-use Vessels. 

The Eppendorf bioprocess solutions have been successfully used in the biopharmaceutical, chemical and nutrition industries as well as applied research for decades.  Our strong synergies in bioreactor technology and polymer manufacturing have helped Eppendorf emerge as a global player and a valuable resource to our customers.

Scalability:  Fermentation process scale-up made easy using the simple methods and techniques that you can apply to your process today.
The ultimate goal for process development is the realization of commercial production. Currently, the scale-up of fermentation processes, which is critical to the success of industrial fermentation for bioproduction, is receiving much attention. The fermentation scale-up from small scale/benchtop (1 L to 10 L scale) to pilot/production scale (100 L) is an important step for effective production of biologicals, including small proteins in the biopharmaceutical market. Engineering parameters critical for scaling-up fermentation processes will be discussed such as the importance of vessel and impeller geometries, tip speed, oxygen transfer rate (OTR), and power number (Np).  Real life examples of scale-up by OTR, constant tip speed and constant power per volume will be illustrated using a model which includes a 1L, 10L, and 100L fermentation systems.        

Case Studies Illustrating Cell Culture Scale-Up Using Stirred-Tank Single-Use Bioreactors
For cultivation of mammalian cells in biopharmaceutical research and manufacturing, single-use technology possesses several advantages to autoclavable material. Bioreactor scalability is critical to streamlining the adaptation of culture volumes during process development and manufacturing.  We will discuss several scaling approaches, such as constant tip speed, k_La, and power number-based scale-up. Data will be highlighted with two case studies.

• First, we determined the power numbers of Eppendorf BioBLU® Single-Use Bioreactors using computational fluid dynamics simulations. Based on these data, we scaled up a process for production of monoclonal antibodies (mAb) in CHO cells from 0.25 L to 3.75 L to 40 L by keeping constant P/V values (impeller power consumption per liquid volume) among the differently sized vessels. Similar cell growth curves and mAb production profiles were achieved at all three scales; and

• Second, we show experimental data from our German customer CEVEC®. Using a scale-up strategy based on constant gas flow rates, power input and pH, the process engineers at CEVEC developed a bioprocess for production of lentiviral vectors or recombinant Adeno-Associated Viruses (rAAVs).

Lunch will be served.

Presenters

Register Now! For more information please contact Carol Stanton at (508) 740-8055 or email her at stanton.c@eppendorf.com.

Participation is free but space is limited so please be sure to click on the "Register Now!" button to register for the event and reserve your spot.  We look forward to seeing you!